Agilent rna pico manual

 · The Agilent Bioanalyzer is marked with this symbol when the user should refer to the Site Preparation and Safety Manual in order to protect the Agilent Bioanalyzer against damage. The Bioanalyzer Small RNA assay provides a fast and sensitive analysis to resolve small nucleic acid samples in the size range of 6 to nt. The Agilent Bioanalyzer is a microfluidics-based platform for sizing, quantification and quality control of DNA, RNA, proteins and cells. The Agilent Bioanalyzer (Nano and Pico) uses chip-based electrophoresis to examine the 18S and 28S ribosomal RNA peaks to determine the amount of degradation in the RNA sample. The Agilent bioanalyzer was subsequently employed, in conjunction with the RNA Pico assay, to determine the quantity and quality of 1 µL (–5 ng) of total RNA.

Agilent RNA Pico Reagents (reorder number ) (blue) RNA Pico Dye Concentrate 1 1 “This product is provided under an agreement between Molecular Probes, Inc. (a wholly owned. 1 Allow the RNA dye concentrate (blue) to equilibrate to room temperature for 30 min. 2 Vortex RNA dye concentrate (blue) for 10 s, spin down and add 1 μL of dye into a 65 μL aliquot of filtered gel. 3 Vortex solution well. Spin tube at g for 10 min at room temperature. Use prepared gel-dye mix within one day. Loading the Gel-Dye Mix. The Agilent Bioanalyzer is a microfluidics-based platform for sizing, quantification and quality control of DNA, RNA, proteins and cells. The Agilent Bioanalyzer (Nano and Pico) uses chip-based electrophoresis to examine the 18S and 28S ribosomal RNA peaks to determine the amount of degradation in the RNA sample.

as governed by. United States and international copyright laws. Manual Part Number. G Rev. C. Edition. 12/ The Ambion RNA Ladder is a set of six RNA transcripts with lengths of , , , The RNA Ladder is designed for use with Agilent's lab-on. SMARTer® Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian User Manual. () G. Protocol: Validation Using the Agilent Bioanalyzer.